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Updated: Apr 12, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
A tetraphenylimidazole-based fluorescent probe for the detection of hydrogen sulfide and its application in living
Biao Gu1, Naxiu Mi1, Youyu Zhang1
1Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Abstract:
A novel probe based on the fluorescence off-on strategy was prepared to optically detect hydrogen sulfide (H2S) via an excited state intramolecular proton transfer (ESIPT) mechanism. The probe shows high sensitivity and excellent selectivity to H2S. It also displays a large Stokes shift (∼140 nm) and a remarkable quantum yield enhancement (Ф=0.412) after interaction with H2S. Moreover, the cellular imaging experiment demonstrated that it has potential utility for H2S sensing in biological sciences.
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