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Published on: June 13, 2016
Expression profile of circulating serum microRNAs in dogs with lymphoma
Aki Fujiwara-Igarashi1, Hirotaka Igarashi2, Noriyuki Mizutani2
1Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; Division of Therapeutic Science I, Department of Clinical Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 183-0023, Japan.
Abstract:
Serum microRNAs (miRNAs) are mediators of cell-to-cell communication and alter the cellular microenvironment; they are stable for hours under certain conditions in body fluids despite the presence of RNases. Certain miRNAs have been found to be altered in the serum or plasma of humans with various cancers and may represent promising, non-invasive biomarkers for various diseases in humans and animals. The objective of this study was to determine the expression profile of circulating miRNAs in the serum of dogs with lymphoma. Serum samples were obtained from 61 dogs with lymphoma and 40 control dogs, and real-time reverse transcription-polymerase chain reaction was used for miRNA measurement. In order to select candidate genes, a comprehensive expression analysis was undertaken prior to validation of several candidate miRNAs. Of 277 miRNAs, five (let-7b, miR-223, miR-25, miR-92a, and miR-423a) were selected as candidates. The expression levels of four miRNAs (let-7b, miR-223, miR-25, miR-92a) were significantly reduced in the lymphoma group, whereas miR-423a levels were significantly increased compared to the controls. When the lymphoma cases were categorized into high- or low-grade as well as into their anatomic form, miR-25 levels were lower in the serum samples from the lymphoma group compared to those from the control group. Although the biological function of serum miRNAs still remains unclear, determining their functional roles in serum and tissues will contribute not only to the identification of potential biomarkers but also to the elucidation of the pathogenesis of canine lymphoma.
Insights
Serum microRNAs (miRNAs) show altered expression in dogs with lymphoma, with four key miRNAs decreasing and one increasing. These findings suggest potential non-invasive biomarkers for canine lymphoma diagnosis and understanding its pathogenesis.
Area of Science:
- Veterinary Medicine
- Molecular Biology
- Oncology
Background:
- Serum microRNAs (miRNAs) are stable molecules involved in cell communication and can be altered in various cancers.
- Altered circulating miRNAs show potential as non-invasive biomarkers for human and animal diseases.
- Canine lymphoma is a common cancer, and identifying reliable biomarkers is crucial for diagnosis and treatment.
Purpose of the Study:
- To investigate the expression profile of circulating miRNAs in the serum of dogs diagnosed with lymphoma.
- To identify specific miRNAs that are differentially expressed in canine lymphoma patients compared to healthy controls.
- To explore the potential of these miRNAs as diagnostic biomarkers for canine lymphoma.
Main Methods:
- Serum samples were collected from 61 dogs with lymphoma and 40 control dogs.
- Real-time reverse transcription-polymerase chain reaction (RT-PCR) was employed for quantitative miRNA measurement.
- A comprehensive expression analysis was performed to select candidate miRNAs, followed by validation.
Main Results:
- Out of 277 analyzed miRNAs, five candidates (let-7b, miR-223, miR-25, miR-92a, and miR-423a) were identified.
- The expression of let-7b, miR-223, miR-25, and miR-92a was significantly decreased in dogs with lymphoma.
- miR-423a levels were significantly increased in the lymphoma group compared to controls.
Conclusions:
- Specific circulating miRNA profiles, including decreased let-7b, miR-223, miR-25, miR-92a and increased miR-423a, are associated with canine lymphoma.
- These miRNAs hold promise as potential non-invasive biomarkers for canine lymphoma.
- Further research into the functional roles of these serum miRNAs could elucidate the pathogenesis of canine lymphoma.
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