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Sensitive fluorescent assay for copper (II) determination in aqueous solution using copper-specific ssDNA and Sybr
Shenshan Zhan1, Hanchu Xu1, Weilin Zhang1
1School of Environmental Science and Engineering; Key Laboratory of Urban Agriculture (South), Ministry of Agriculture; School of Agriculture and Biology, and Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, PR China.
Abstract:
This paper reports a fluorescent turn-off assay for sensitive detection of Cu(2+) in an aqueous solution by using a copper-specific ssDNA Cu100 and Sybr Green I. By monitoring the fluorescence changes arose from different interactions of Sybr Green I with Cu100 and Cu100/Cu(2+) complex, the Cu(2+) could be linearly detected from 5.57 to 250 ppb, with a detection limit of 5.57 ppb. The feasibility of this assay was demonstrated by detecting Cu(2+) in certified reference materials and spiked water samples with satisfactory results.
