Related Experiment Videos
Differentiation between Trichinella spiralis and T. pseudospiralis infective larvae by a monoclonal antibody
J Rodriguez-Perez1, V Gomez-Garcia, M Rodriguez-Osorio
1Instituto López-Neyra de Parasitología, Unidad de Inmunología, Granada, Spain.
Abstract:
Crude saline extracts of Trichinella spiralis and T. pseudospiralis infective larvae were studied by Western blot analysis using a monoclonal antibody, named ES/TA2 and produced against T. spiralis larvae. This monoclonal antibody recognized seven major antigenic components in T. spiralis larvae with apparent Mr: 45, 48, 50, 68, 70, 92 and 105 kDa and five in T. pseudospiralis larvae: 38, 50, 70, 72 and 92 kDa. SDS-PAGE of both extracts did not reveal appreciable differences in the range of molecular weights recognized by ES/TA2. These facts show the existence of immunological differences among proteins with apparently identical molecular weights.