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Evaluating the Immune Response of a Nanoemulsion Adjuvant Vaccine Against Methicillin-Resistant Staphylococcus aureus MRSA Infection
Published on: September 1, 2023
Phospholipid structured microemulsion as effective carrier system with potential in methicillin sensitive
Tanya Chhibber1, Sheetu Wadhwa1, Parul Chadha2
1a University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Studies, Panjab University , Chandigarh , India .
Abstract:
Burn wounds are foremost site for bacterial colonization and multiplication. Staphylococcus aureus is one of the most predominant pathogen found in burn wounds. Fusidic acid (FA) is widely employed in the treatment of complicated skin infections caused by Staphylococcus aureus. Hence, the aim of this study was to investigate the usefulness and efficacy of topical FA (2% w/w) loaded biocompatible microemulsion-based-system (FA-ME) in eradicating MSSA bacterial infections which otherwise was less effective when dealt with conventional formulations. For construction of pseudoternary phase diagram, ratio of oil (IPM):water:Smix is 20:30:50% w/w and proportion of Smix (Phospholipid:Tween 80 (T80):Ethanol) is in the ratio of 1:2:1, respectively. The hypothesis relates here to the role of phospholipids as part of the nano-scale structure of microemulsion systems to overcome the hurdles of drug delivery. The prepared FA-ME system was evaluated for its therapeutic efficacy and carrier-specific characteristics such as globule size, % transmittance, transmission electron microscopy, drug content and stability. Selected microemulsion system was incorporated into gel form and evaluated for texture analysis, drug permeation in 24 h and treatment of burn wounds. Burn wound infection was established with MSSA ATCC 25923 in BALB/c mice and the process of wound healing as well as bacterial loading in the wound was estimated. The developed nanosized FA-ME system demonstrated improved wound healing, better spreadability and enhanced therapeutic efficacy due to the changes in the behavior of the drug molecules by way of carrier-characteristics.
Insights
A novel topical fusidic acid microemulsion system (FA-ME) effectively treats Staphylococcus aureus burn wound infections. This nanocarrier enhances drug delivery and improves wound healing compared to conventional formulations.
Area of Science:
- Pharmaceutical Sciences
- Biotechnology
- Dermatology
Background:
- Burn wounds are susceptible to bacterial colonization, with Staphylococcus aureus being a common pathogen.
- Fusidic acid (FA) is a key treatment for Staphylococcus aureus infections, but conventional formulations show limited efficacy.
- Developing advanced drug delivery systems is crucial for overcoming challenges in treating topical infections.
Purpose of the Study:
- To develop and evaluate a biocompatible topical fusidic acid microemulsion-based system (FA-ME) for treating Methicillin-Susceptible Staphylococcus aureus (MSSA) burn wound infections.
- To investigate the therapeutic efficacy and carrier-specific characteristics of the FA-ME system.
- To compare the efficacy of FA-ME with conventional formulations in an in vivo burn wound model.
Main Methods:
- Formulation of a pseudoternary phase diagram to optimize microemulsion composition (oil, water, Smix).
- Characterization of the FA-ME system including globule size, % transmittance, transmission electron microscopy, drug content, and stability.
- Incorporation of FA-ME into a gel formulation for evaluation of texture, drug permeation, and in vivo burn wound healing in BALB/c mice infected with MSSA.
Main Results:
- The developed nanosized FA-ME system exhibited favorable physicochemical properties and stability.
- FA-ME demonstrated enhanced therapeutic efficacy in treating MSSA burn wound infections in mice.
- The microemulsion system improved wound healing processes and reduced bacterial load compared to conventional treatments.
Conclusions:
- Topical FA-ME is a promising nanocarrier system for effective treatment of Staphylococcus aureus burn wound infections.
- The microemulsion formulation enhances fusidic acid delivery, leading to improved wound healing and antimicrobial activity.
- This advanced system offers a potential alternative to conventional treatments for complicated skin infections.
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