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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

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Rotavirus

Mathew D Esona1, Rashi Gautam1

  • 1Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, Northeast, Mail Stop G04, Atlanta, GA 30333, USA.

Clinics in Laboratory Medicine
|May 26, 2015
PubMed

Insights

Group A rotavirus (RVA) causes severe gastroenteritis in children. New molecular assays offer sensitive detection and characterization of RVA strains, aiding in patient treatment and monitoring of vaccine and wild-type strains.

Area of Science:

  • Virology
  • Pediatrics
  • Molecular Diagnostics

Background:

  • Group A rotavirus (RVA) is a primary cause of acute gastroenteritis (AGE) in young children globally.
  • The widespread use of Rotarix® and RotaTeq® vaccines has significantly decreased RVA-related AGE and mortality.
  • Accurate diagnosis and strain characterization are crucial for patient management and epidemiological surveillance.

Purpose of the Study:

  • To highlight the need for advanced diagnostic techniques for rotavirus.
  • To introduce new molecular assays for rotavirus detection and characterization.
  • To compare the sensitivity and specificity of novel assays against conventional methods.

Main Methods:

  • Development and validation of new molecular assays.
  • Application of high-throughput techniques for rotavirus detection.
  • Genotyping and full genome characterization of rotavirus strains.

Main Results:

  • New molecular assays demonstrate enhanced sensitivity and specificity compared to conventional methods.
  • These assays enable rapid detection and comprehensive characterization of rotavirus strains.
  • The techniques can differentiate between wild-type and vaccine strains (Rotarix®, RotaTeq®).

Conclusions:

  • Novel molecular assays are essential for accurate and timely diagnosis of rotavirus infections.
  • These advanced methods improve the monitoring of circulating rotavirus strains, including vaccine and wild-type.
  • The development of sensitive and specific assays supports effective public health strategies against rotavirus gastroenteritis.

Keywords:
Acute gastroenteritisMultiplex assaysNext-generation sequencingRotaTeq®Rotarix®RotavirusRotavirus vaccinesqRT-PCR

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