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Updated: Apr 12, 2026
Colony Forming Cell CFC Assay for Human Hematopoietic Cells
Published on: December 18, 2010
NUP98/11p15 translocations affect CD34+ cells in myeloid and T lymphoid leukemias
Barbara Crescenzi1, Valeria Nofrini1, Gianluca Barba1
1Laboratory of Molecular Medicine, CREO, University of Perugia and A.O. Perugia, 06132 Perugia, Italy.
Abstract:
We assessed lineage involvement by NUP98 translocations in myelodysplastic syndromes (MDS), acute myeloid leukaemia (AML), and T-cell acute lymphoblastic leukaemia (T-ALL). Single cell analysis by FICTION (Fluorescence Immunophenotype and Interphase Cytogenetics as a Tool for Investigation of Neoplasms) showed that, despite diverse partners, i.e. NSD1, DDX10, RAP1GDS1, and LNP1, NUP98 translocations always affected a CD34+/CD133+ hematopoietic precursor. Interestingly the abnormal clone included myelomonocytes, erythroid cells, B- and T- lymphocytes in MDS/AML and only CD7+/CD3+ cells in T-ALL. The NUP98-RAP1GDS1 affected different hematopoietic lineages in AML and T-ALL. Additional specific genomic events, were identified, namely FLT3 and CEBPA mutations in MDS/AML, and NOTCH1 mutations and MYB duplication in T-ALL.
