Related Experiment Video
Updated: Apr 12, 2026

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants
Published on: May 1, 2013
Peptidoglycan diversity and anti-inflammatory capacity in Lactobacillus strains
Zhen Wu1, Daodong Pan2, Yuxing Guo1
1Department of Food Science and Nutrition, Jinling College, Nanjing Normal University, Nanjing 210097, Jiangsu, China.
Abstract:
Lactobacillus species are potential probiotic bacteria for humans because of their capacity to improve certain biological functions in the host's immune system. In this study, we focused on three peptidoglycans (PGNs) derived from different Lactobacillus strains and investigated each PGN's anti-inflammatory capacity. Each PGN was analyzed using HPLC, MALDI-TOF/TOF MS and FTIR. All three PGNs displayed a β-1,4-linked N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) structure with some modifications in the polypeptides at the end of the MurNAc residue. In a new insight, we found that PGNs inhibit the release of inflammatory cytokines in LPS-induced RAW 264.7 cells; a capacity that may be related to the TLR-4 pathway. The goal for exploring PGN diversity in Lactobacillus strains is to better understand the potential use of Lactobacillus PGNs in food and pharmaceutical applications.
Related Concept Videos
Inhibitors of Gram-positive Cell Wall Synthesis
Probiotics
Clinical Significance of Antibiotic Resistance
Formation of Lipopolysaccharides
Peptidoglycan Synthesis
Bacterial Phylum Firmicutes

