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Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays
Published on: August 9, 2024
Synthesis and characterization of Se-adenosyl-L-selenohomocysteine selenoxide
Richard I Duclos1, Dillon C Cleary2, Kalli C Catcott2
1Department of Pharmaceutical Sciences, 140 The Fenway Bldg., Room 206, Northeastern University, 360 Huntington Avenue, Boston, Massachusetts 02115-5000, Tel: +1 617 373 3163.
Abstract:
Selenium is an essential micronutrient in humans due to the important roles of the selenocysteine-containing selenoproteins. Organoselenium metabolites are generally found to be substrates for the biochemical pathways of their sulfur analogs, and the redox chemistry of selenomethionine and some other metabolites have been previously reported. We now report the first synthesis and characterization of Se-adenosylselenohomocysteine selenoxide (SeAHO) prepared via hydrogen peroxide oxidation of Se-adenosylselenohomocysteine (SeAH). The selenoxide SeAHO, in contrast to its corresponding sulfoxide S-adenosylhomocysteine (SAHO), can form hydrate, has an electrostatic interaction between the α-amino acid moiety and the highly polar selenoxide functional group, and readily oxidizes glutathione (GSH) and cysteine thiols.
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