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Heterogeneous Electrochemical Aptamer-Based Sensor Surfaces for Controlled Sensor Response.

Lauren R Schoukroun-Barnes¹, Ethan P Glaser¹, Ryan J White¹

¹Department of Chemistry and Biochemistry, University of Maryland Baltimore County (UMBC), 1000 Hilltop Circle, Baltimore, Maryland 21250, United States.

Langmuir : the ACS Journal of Surfaces and Colloids

|May 26, 2015

Summary

This summary is machine-generated.

Researchers developed a new method to tune the performance of electrochemical aptamer-based sensors. By mixing aptamers with varying affinities, they precisely controlled sensor sensitivity and dynamic range for detecting small molecules.

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Area of Science:

Biosensors and Nanotechnology
Molecular Recognition and Aptamer Engineering

Background:

Structure-switching sensors rely on conformational changes in recognition elements for signal transduction.
Electrochemical aptamer-based (EAB) sensors detect analytes by measuring electron transfer changes due to aptamer conformational shifts.
The analytical performance of EAB sensors is directly influenced by the magnitude of aptamer conformational changes.

Purpose of the Study:

To establish a generalizable method for predictably tuning the sensitivity and linear range of EAB sensors.
To achieve predictable control over sensor performance by employing mixtures of rationally designed aptamer sequences targeting the same analyte but with differing affinities.

Main Methods:

Development of heterogeneous EAB sensors on a single electrode surface using mixtures of aptamer sequences.
Design of aptamers with specific affinities for target analytes (adenosine triphosphate and tobramycin).
Characterization of sensor performance using electrochemical techniques and analysis via a bi-Langmuir-type isotherm model.

Main Results:

Demonstrated successful tuning of sensor affinity, dynamic range, and sensitivity by employing mixtures of modified aptamer sequences.
Validated the predictability of sensor performance modulation using a bi-Langmuir-type isotherm.
Successfully developed heterogeneous sensors for small molecule targets like adenosine triphosphate and tobramycin.

Conclusions:

Mixtures of aptamer sequences with varying affinities offer a powerful strategy for predictable optimization of EAB sensor analytical performance.
This approach provides a generalizable method to tailor sensor sensitivity and linear range for diverse small molecule detection applications.
The bi-Langmuir-type isotherm effectively predicts the performance of these heterogeneous aptamer-based sensors.