Ammonium fluoride as a mobile phase additive in aqueous normal phase chromatography
Joseph J Pesek1, Maria T Matyska1
1Department of Chemistry, San Jose State University, San Jose, CA 95192, USA.
Journal of Chromatography. A
|May 27, 2015
Summary
Ammonium fluoride shows potential as a mobile phase additive in chromatography, offering comparable performance to standard additives but with concerns about column longevity. Further research is needed to assess its long-term impact.
Area of Science:
- Analytical Chemistry
- Chromatography
- Mass Spectrometry
Background:
- Aqueous normal phase chromatography (ANPC) is a valuable technique for analyzing polar compounds.
- Silica hydride stationary phases offer unique selectivity in ANPC.
- Standard mobile phase additives like formic acid and ammonium formate are commonly used.
Purpose of the Study:
- To evaluate ammonium fluoride as a novel mobile phase additive in ANPC.
- To compare its performance against established additives (formic acid, ammonium formate).
- To assess its impact on column parameters and lifetime.
Main Methods:
- ANPC coupled with mass spectrometry detection was employed.
- Ammonium fluoride was tested as a mobile phase additive with silica hydride columns.
- Performance was evaluated using a panel of test solutes (e.g., NAD, AMP, ATP).
- Parameters assessed included retention times, peak shape, efficiency, and intensity in positive and negative ion modes.
Main Results:
- Ammonium fluoride demonstrated comparable retention times, peak shape, efficiency, and intensity to standard additives.
- The additive's performance was assessed across various test analytes.
- Column reproducibility was measured, and the effects of switching additives were investigated.
Conclusions:
- Ammonium fluoride is a viable alternative mobile phase additive in ANPC for specific applications.
- Potential concerns exist regarding its aggressive nature and impact on column lifetime.
- Further studies are warranted to fully understand its long-term effects and optimize its use.
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