Related Experiment Video
Updated: Apr 12, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
Published on: November 23, 2016
TPC: the NAADP discovery channel?
Anthony J Morgan1, Lianne C Davis1, Margarida Ruas1
1*Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, U.K.
Abstract:
The Ca2+-mobilizing second messenger, NAADP (nicotinic acid adenine dinucleotide phosphate), has been with us for nearly 20 years and yet we still cannot fully agree on the identity of its target Ca2+-release channel. In spite of some recent robust challenges to the idea that two-pore channels (TPCs) represent the elusive "NAADP receptor", evidence continues to accumulate that TPCs are important for NAADP-mediated responses. This article will briefly outline the background and review more recent work pertaining to the TPC story.
Related Concept Videos
The ADP/ATP Carrier Protein
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
Nuclear Protein Sorting
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
NMR Spectroscopy Of Amines
Proton (¹H) NMR: Chemical Shift
Absorption signals of all the protium nuclei...
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

