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Updated: Apr 12, 2026

Introduction to Solid Supported Membrane Based Electrophysiology
Published on: May 11, 2013
The Hill analysis and co-ion-driven transporter kinetics
Juke S Lolkema1, Dirk-Jan Slotboom1
1Department of Molecular Microbiology and Department of Membrane Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, Netherlands d.j.slotboom@rug.nl j.s.lolkema@rug.nl.
Abstract:
Interaction of multiple ligands with a protein or protein complex is a widespread phenomenon that allows for cooperativity. Here, we review the use of the Hill equation, which is commonly used to analyze binding or kinetic data, to analyze the kinetics of ion-coupled transporters and show how the mechanism of transport affects the Hill coefficient. Importantly, the Hill analysis of ion-coupled transporters can provide the exact number of transported co-ions, regardless of the extent of the cooperativity in ion binding.
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