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Updated: Apr 11, 2026
Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
Published on: March 16, 2017
A water-soluble fluorescent pH probe based on perylene dyes and its application to cell imaging
Yongshan Ma1, Fengxia Zhang2, Jinfeng Zhang1
1School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan, 250101, People's Republic of China.
Abstract:
A fluorescent pH probe, N,N'-bi( l-phenylalanine amine)-perylene-3,4;9,10-dicarboxylic diimide (PDCDA) was synthesized and used for pH sensing in living cells. A significant fluorescence intensity change was observed over a pH range from 7.0 to 4.0. Electrostatic potential maps (MEP) suggested that the electronic repulsion between PDCDAs was increased by the high negative electrostatic potential which resulted in a high water solubility of PDCDA. PDCDA was successfully applied as a high-performance fluorochrome for living HeLa cell imaging. The results demonstrate that the probe PDCDA is a good candidate for monitoring pH fluctuations in living cells with good water solubility, low cytotoxicity, high fluorescence quantum yield and photostability.
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