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[Spleen and hematopoiesis].

M Mori, H Kurata

    [Rinsho Ketsueki] the Japanese Journal of Clinical Hematology
    |August 1, 1989
    PubMed
    Summary
    This summary is machine-generated.

    The human spleen plays a role in hematopoiesis, with fetal spleens containing hematopoietic stem cells. Adult spleen conditioned media stimulates the growth of certain blood precursor cells, indicating a potential regulatory function.

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    Area of Science:

    • Hematology
    • Immunology
    • Developmental Biology

    Background:

    • The spleen's role in hematopoiesis, particularly in adults, is not fully understood.
    • Hematopoietic stem cells (HSCs) are crucial for blood cell formation.
    • Previous research has focused on the spleen's role in immune responses and filtering blood.

    Purpose of the Study:

    • To investigate the presence and function of hematopoietic stem cells in fetal and adult human spleens.
    • To evaluate the stimulatory effects of adult spleen conditioned media on hematopoietic progenitor cells.
    • To examine the expression of key hematopoietic growth factors in fetal and adult spleens.

    Main Methods:

    • Evaluation of BFU-E (burst-forming unit-erythroid) and CFU-C (colony-forming unit-culture) in fetal and adult spleen samples.

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  • Testing conditioned media from adult spleen cells for stimulatory activity on bone marrow mononuclear cells.
  • Immunohistochemical analysis of fetal and adult spleens using antibodies against G-CSF, GM-CSF, and erythropoietin.

    • Main Results:

    • BFU-E and CFU-C were detected in 20 and 23-week fetal spleens.
    • Adult spleens contained few stem cells, possibly due to peripheral blood contamination.
    • Adult spleen conditioned media stimulated in vitro growth of BFU-E and CFU-C; PHA-stimulated media augmented BFU-E but suppressed CFU-C.
    • G-CSF and GM-CSF were found in fetal spleens, while only GM-CSF was detected in adult spleens.

    Conclusions:

    • Fetal spleens harbor hematopoietic stem cells and express G-CSF and GM-CSF.
    • Adult spleen conditioned media possesses stimulatory activity for hematopoietic progenitor cells, suggesting a supportive role in hematopoiesis.
    • The spleen's hematopoietic function may differ between fetal and adult stages, with GM-CSF being a key factor in adults.