Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.

Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Correction: Family caregiving as a pathway to strengthen health outcomes in India.

Frontiers in public health·2026

Same author

Phase transition dynamics of HBDBA-MWCNT nanocomposites probed by temperature-dependent Raman spectroscopy.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026

Same author

Family caregiving as a pathway to strengthen health outcomes in India.

Frontiers in public health·2025

Same author

Major pathophysiological changes in pulmonary disease provided a molecular insight based on deep learning approach.

Scientific reports·2025

Same author

Unraveling coalification dynamics: a comprehensive spectroscopic study on the chemical and microstructural evolution from lignite to semi-anthracite.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2025

Same author

FDA Approval Summary: Idecabtagene Vicleucel for the Treatment of Triple-Class-Exposed, Relapsed or Refractory Multiple Myeloma.

Clinical cancer research : an official journal of the American Association for Cancer Research·2025

Search research articles

Related Experiment Video

Updated: Apr 11, 2026

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

Temperature dependent Raman and DFT study of creatine.

Debraj Gangopadhyay¹, Poornima Sharma¹, Ranjan K Singh¹

¹Department of Physics, Banaras Hindu University, Varanasi 221005, India.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy

|May 27, 2015

Summary

Investigating creatine powder using Raman spectroscopy and density functional theory (DFT) revealed temperature-dependent changes in its crystal structure. Cooling creatine induces phase transitions and the formation of higher hydrogen-bonded aggregates.

Keywords:

Creatine DFT Hydrogen bonding Temperature dependent Raman spectroscopy

More Related Videos

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

Related Experiment Videos

Last Updated: Apr 11, 2026

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

Area of Science:

Solid-state chemistry
Spectroscopy
Computational chemistry

Background:

Creatine is a biomolecule with potential applications in medicine and sports.
Understanding its crystal structure and phase transitions is crucial for its effective utilization.
Temperature significantly influences molecular interactions and crystal lattice dynamics.

Purpose of the Study:

To investigate the effect of temperature on the hydrogen-bonded network in creatine crystals.
To analyze the vibrational properties and phase transitions of creatine powder.
To correlate experimental spectroscopic data with theoretical calculations.

Main Methods:

Temperature-dependent Raman spectroscopy was performed on creatine powder from 420 K to 100 K.
Density functional theory (DFT) calculations were used to optimize creatine clusters and assign vibrational modes.
Potential energy distribution (PED) analysis was employed to understand intermolecular interactions.

Main Results:

Raman spectra showed clear signatures of a phase transition between 200 K and 180 K.
DFT calculations aided in assigning 48 normal modes of the zwitterionic form of creatine.
Analysis revealed temperature-dependent thermal motion and changes in intermolecular interactions within the crystal lattice.

Conclusions:

Cooling creatine leads to the formation of higher hydrogen-bonded aggregates.
A distinct phase transition occurs in creatine crystals between 180 K and 200 K.
The study provides insights into the temperature-dependent behavior of creatine's crystal structure and hydrogen bonding.