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[Fundamental examination and the reference values of anticardiolipin antibodies]
Insights
This study establishes reference values for anticardiolipin antibodies using ELISA in healthy individuals. The enzyme-linked immunosorbent assay is effective for diagnosing autoimmune disorders, despite potential interferences.
Area of Science:
- Immunology
- Clinical Chemistry
Context:
- Anticardiolipin antibodies are associated with various autoimmune diseases.
- Accurate reference values are crucial for reliable diagnostic assays.
Purpose:
- To establish reference values for anticardiolipin antibodies in a healthy population using ELISA.
- To evaluate the performance and identify potential interferences of the ELISA method for anticardiolipin antibody detection.
Summary:
- Sera from 146 healthy individuals were analyzed to determine reference values for anticardiolipin antibodies (aCL) via ELISA.
- The ELISA method demonstrated good reproducibility and stability but showed interference from triglycerides, hemoglobin, and bilirubin.
- Positive rates for IgG, IgA, and IgM aCL in healthy individuals were determined, with a notably high incidence of IgG aCL.
Impact:
- Provides essential reference data for the clinical use of ELISA in detecting anticardiolipin antibodies.
- Highlights potential interfering substances that require consideration during assay interpretation.
- Supports the utility of ELISA for diagnosing and monitoring systemic lupus erythematosus and related autoimmune conditions.
Abstract:
We studied sera from 146 healthy individuals in a fundamental examination to obtain reference values for anticardiolipin antibodies by the enzyme-linked immunosorbent assay (ELISA). The good results were obtained in the evaluation of concentrations of pretreatment substances, cardiolipin antigen and serum samples, as well as in reproducibility and stability on the storage of the samples. However, there was interference by triglycerides, hemoglobin and birilubin. The O.D. values and positive values of IgG, IgA and IgM in healthy individuals were 0.105, 0.111, 0.173 and 4.1%, 2.7%, 2.7% respectively. Furthermore, the incidences of anticardiolipin antibodies in systemic lupus erythematosus, mixed connective tissue disease, progressive systemic sclerosis, Sjogren's syndrome, polymyositis/dermatomyositis, rheumatoid arthritis, unclassified connective tissue disease, polyartertis nodosa and idiopathic thrombocytopenic purpura were 48.0%, 40.0%, 50.0%, 21.4%, 26.3%, 29.4%, 40.0% and 25.0% respectively. Especially, the incidence of IgG antibody against cardiolipin was high. Detection of phospholipid cardiolipin antibodies using ELISA was considered to be a useful for clinical diagnosis and monitoring of patients with systemic lupus erythematosus and associated autoimmune disorders.