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Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
Published on: July 6, 2021
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Mammalian designer cells: Engineering principles and biomedical applications.
Mingqi Xie1, Martin Fussenegger2,3
1Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.
Biotechnology Journal
|May 27, 2015
Summary
Synthetic biology engineers designer cells for advanced biotechnology applications. These reprogrammed mammalian cells offer precise control for biopharmaceutical manufacturing and novel cell-based therapies.
Area of Science:
- Biotechnology and Synthetic Biology
- Interdisciplinary applications in medicine, agriculture, and manufacturing
Background:
- Synthetic biology advances biotechnology by engineering novel biological systems.
- Designer cells offer controllable behavior for specific applications.
Purpose of the Study:
- To review techniques for engineering mammalian designer cells.
- To highlight control mechanisms and applications of designer cells.
Main Methods:
- Engineering mammalian cells with controlled gene expression (transcriptional and translational levels).
- Coupling cellular functions to extracellular stimuli for remote control.
- Utilizing user-defined trigger signals for precise regulation.
Main Results:
- Achieved kinetic and dynamic control over target gene expression.
- Enabled remote control of designer cell behavior via external signals.
- Demonstrated potential for improved biopharmaceutical production and cell-based therapies.
Conclusions:
- Engineered mammalian designer cells offer enhanced biological functions.
- These cells are crucial for advancing biopharmaceutical manufacturing and translational medicine.
- Future applications include complex tissue and organism reprogramming.
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