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Updated: Apr 11, 2026

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
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Published on: March 13, 2018

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Prospective cohort study with active surveillance for fever in four dengue endemic countries in Latin America

Gustavo Dayan, Jose L Arredondo, Gabriel Carrasquilla

    The American Journal of Tropical Medicine and Hygiene
    |May 28, 2015
    PubMed

    Insights

    This study assessed dengue incidence in children preparing for a vaccine trial. Results show sites can detect dengue cases and febrile episodes, supporting future efficacy studies.

    Area of Science:

    • * Clinical research
    • * Infectious diseases
    • * Vaccinology

    Background:

    • * Preparation for a Phase III dengue vaccine efficacy trial required understanding local dengue incidence.
    • * An observational study was conducted in a cohort of children aged 9-16 years.

    Purpose of the Study:

    • * To identify dengue infections within a defined pediatric cohort.
    • * To evaluate the capability of investigational sites in detecting dengue cases and febrile episodes.

    Main Methods:

    • * Observational study involving 3,000 children aged 9-16 years across 20 sites.
    • * Monitoring of acute febrile episodes and serological/virological confirmation of dengue.
    • * Calculation of incidence density for febrile episodes and dengue cases.

    Main Results:

    • * 50 probable dengue cases (21.3%) and 18 confirmed dengue cases (7.7%) were identified from 255 febrile episodes.
    • * Overall incidence density of acute febrile episodes was 17.7 per 100 person-years.
    • * Incidence varied by location, from 15.3 in Colombia to 22.0 in Puerto Rico.

    Conclusions:

    • * Investigational sites demonstrated capacity to capture and follow acute febrile episodes.
    • * The study successfully detected dengue cases, validating site readiness for the Phase III trial.
    • * Findings provide crucial epidemiological data for dengue vaccine efficacy assessment.

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