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Updated: Apr 11, 2026

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

DNA-Encoded Dynamic Combinatorial Chemical Libraries.

Francesco V Reddavide¹, Weilin Lin¹, Sarah Lehnert¹

¹B CUBE Center for Molecular Bioengineering, Technische Universität Dresden, Dresden 01307 (Germany).

Angewandte Chemie (International Ed. in English)

|May 28, 2015

Summary

This summary is machine-generated.

DNA-encoded dynamic combinatorial chemical libraries (EDCCLs) enhance protein binder discovery. This novel approach improves signal-to-noise and enriches potent bivalent binders through in situ synthesis.

Keywords:

DNA-encoded chemical libraries DNA-templated reactions drug discovery dynamic combinatorial chemistry protein-ligand interactions

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Last Updated: Apr 11, 2026

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

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Published on: January 14, 2020

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Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Published on: September 6, 2012

Area of Science:

Chemical biology
Drug discovery
Molecular recognition

Background:

Dynamic combinatorial chemistry (DCC) studies reversible reactions but faces challenges in analyzing complex mixtures for protein binder discovery.
DNA-encoded chemical library (DECL) technology enables screening of vast compound libraries but often suffers from low signal-to-noise ratios and poor affinity correlation.

Purpose of the Study:

To design and apply DNA-encoded dynamic combinatorial chemical libraries (EDCCLs) to overcome limitations in current binder discovery methods.
To enhance the discovery of high-affinity protein binders, particularly bivalent binders.

Main Methods:

Development and implementation of the EDCCL approach, integrating DCC principles with DECL technology.
Utilizing DNA-templated chemical reactions within the EDCCL framework.

Main Results:

EDCCLs successfully convert monovalent binders into high-affinity bivalent binders.
Remarkably enhanced enrichment of potent bivalent binders was achieved by driving their in situ synthesis.
Demonstrated the utility of EDCCLs in DNA-templated chemical reactions.

Conclusions:

EDCCLs offer a powerful strategy to improve the efficiency and effectiveness of protein binder discovery.
This technology enhances the identification of high-affinity bivalent binders and facilitates DNA-templated synthesis.