Multiple MoS2 Transistors for Sensing Molecule Interaction Kinetics.

Area of Science:

• Materials Science
• Nanotechnology
• Biomedical Engineering

Background:

• Atomically layered transition metal dichalcogenides (TMDCs) offer potential for next-generation biosensors.
• Device calibration is crucial for quantifying biomolecule interactions using TMDC-based sensors.

Purpose of the Study:

• To demonstrate MoS2 transistor biosensors for detecting tumor necrosis factor-alpha (TNF-α).
• To calibrate sensor responses for quantifying biomolecule interaction affinities and kinetics.

Main Methods:

• Fabrication of MoS2-based transistor biosensors.
• Detection of TNF-α in both linear and subthreshold transistor regimes.
• Calibration of sensor responses to establish a standard curve.

Main Results:

• Achieved a detection limit as low as 60 fM for TNF-α.
• Consistent calibrated responses across different transistor regimes.
• Extracted equilibrium constant (KD = 369 ± 48 fM) and binding kinetics for antibody-TNF-α interactions.

Conclusions:

• MoS2 transistor biosensors can achieve ultra-low detection limits for specific biomolecules.
• The study provides a calibrated model for analyzing biomolecular interactions with high sensitivity.
• This work advances TMDC-based biosensing for fM-level biomolecule interaction analysis.