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Automated cell viability assessment using a microfluidics based portable imaging flow analyzer
Veerendra Kalyan Jagannadh1, Jayesh Vasudeva Adhikari1, Sai Siva Gorthi1
1Optics and Microfluidics Instrumentation Lab, Department of Instrumentation and Applied Physics, Indian Institute of Science , Bangalore 560012, India.
Biomicrofluidics
|May 28, 2015
Summary
This study presents a portable optofluidic imaging flow analyzer using a cellphone and microfluidics, achieving high throughput for cell analysis and automated viability assessment.
Area of Science:
- Biomedical Engineering
- Microfluidics
- Optical Imaging
Background:
- High-throughput cell analysis is crucial for diagnostics and research.
- Existing systems are often bulky and expensive.
- Portable, cost-effective solutions are needed for broader accessibility.
Purpose of the Study:
- To develop a portable optofluidic imaging flow analyzer.
- To achieve high throughput using microfluidics and cellphone-based imaging.
- To demonstrate automated cell viability assessment and blood cell counting.
Main Methods:
- System-level integration of portable microscopy and microfluidics.
- Utilizing a cellphone augmented with off-the-shelf optical components.
- Employing custom-designed microfluidics with multiple channels for enhanced throughput.
Main Results:
- Demonstrated a portable optofluidic imaging flow analyzer with a throughput of 450 cells/s.
- Successfully imaged yeast cells and performed automated cell viability assessment.
- Showcased suitability for blood cell counting applications.
Conclusions:
- The developed system offers a portable and high-throughput solution for cell analysis.
- Cellphone-based imaging combined with microfluidics enables accessible diagnostics.
- The platform shows potential for point-of-care applications and research.
