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Updated: Aug 4, 2026
Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
Published on: December 21, 2014
Cryopreservation effect on proliferation and differentiation potential of cultured chorion cells
Background:
Fetoplacental tissues including the early chorion contain stem cells with various morphological and functional characteristics. Cultured chorionic cells may be used in perspective therapies of different pathologies.
Objective:
To investigate the effect of cryopreservation on proliferation and differentiation potential of chorion cell culture (ChCC).
Materials And Methods:
Five freezing programs for ChCC were compared: Program 1, cooling from 25 degrees C down to -30 degrees C at 0.5 degrees C/min; Program 2, cooling from 25 degrees C down to -30 degrees C at 1 degrees C/min; Program 3, cooling from 25 degrees C down to -10 degrees C at 1 degrees C/min with further cooling down to - 80 degrees C at 10 degrees C/min; Program 4, cooling from 25 degrees C down to -5 degrees C at 1 degrees C/min with further cooling down to -80 degrees C at 10 degrees C/min; Program 5, cooling from 25 degrees C down to -6 degrees C at 1 degrees C/min with further crystal seeding by adding the surplus nitrogen into the chamber, and cooling down to -80 degrees C at 10 degrees C/min. Viability, adhesion, proliferation and directed differentiation were examined.
Results:
Freezing program 5 achieved the best result, with the highest viability, adhesion, proliferation and directed differentiation.
Conclusion:
The data may help establishing better cryopreservation protocols for perspective chorionic cell lines and their further application in biotechnology.
