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Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality
Cristina Tomas1, José Gómez-Fernandez2, Emilio Gómez-Izquierdo2
1Centro de Investigacion y Tecnología Animal - Instituto Valenciano de Investigaciones Agrarias(CITA-IVIA), 12400 Segorbe Castellon, Spain.
Background:
During freezing the selective precipitation of substances in the medium may provoke a pH shift and lead to sperm damage.
Objective:
To study the effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality.
Methods:
A total of 15 ejaculates from different boars were obtained and divided into six aliquots prior to a standard straw cryopreservation in freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) with different pH. After thawing, sperm quality (plasma membrane integrity, motility and acrosome status) were assessed at 30 and 90 minutes of post-thaw incubation at 37 degree C.
Results:
When the boar sperm were frozen in a freezing media with pH basic, and particularly at pH 8, it had higher post-thaw sperm quality.
Conclusion:
The pre-adjustment at pH 8 of the freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) is able to improve the post-thaw boar sperm quality.
