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Quantitative Assay Validation for Oxandrolone in Human Plasma Using LC-MS-MS.
Matthew W Linakis1, Chris Stockmann2, Sarah C Campbell3
1Division of Clinical Pharmacology, Department of Pediatrics, University of Utah, 295 Chipeta Way, Suite 1S100, Salt Lake City, UT 84108, USA Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, 30 S 2000 E Salt Lake City, UT 84112, USA.
A new high-performance liquid chromatography-tandem mass spectrometry (LC-MS-MS) method accurately quantifies oxandrolone in human plasma. This validated assay is crucial for pharmacokinetic studies of the anabolic steroid oxandrolone.
Area of Science:
- Analytical Chemistry
- Pharmacology
Background:
- Oxandrolone is an anabolic steroid used to treat wasting syndromes.
- Accurate quantification of oxandrolone in human plasma is essential for pharmacokinetic studies.
Purpose of the Study:
- To develop and validate a high-performance liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for oxandrolone determination in human plasma.
Main Methods:
- Liquid-liquid extraction of oxandrolone and methyltestosterone (internal standard) from 0.5 mL plasma using n-butyl chloride.
- Reversed-phase HPLC separation on a Luna C18 column with a methanol/ammonium formate buffer mobile phase.
- Quantification using selected reaction monitoring (SRM) on a tandem mass spectrometer.
Main Results:
- A validated LC-MS-MS method for oxandrolone quantification in human plasma was established.
- The method demonstrated suitability for pharmacokinetic studies.
Conclusions:
- This is the first validated LC-MS-MS method for oxandrolone quantification in human plasma.
- The developed assay provides a reliable tool for future oxandrolone pharmacokinetic research.
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