Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nephrons01:10

Nephrons

9.5K
The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma...
9.5K
Nephrotic Syndrome I : Introduction01:24

Nephrotic Syndrome I : Introduction

1.0K
Nephrotic Syndrome is a chronic kidney disorder defined by clinical findings such as severe proteinuria, hypoalbuminemia, hyperlipidemia, and edema. These symptoms result from damage to the glomeruli, the kidney’s filtering units, increasing their permeability to proteins.Definition and Meaning:Proteinuria, defined as the loss of more than 3.5 grams of protein per day in adults, is a crucial feature of nephrotic syndrome. This condition is often accompanied by edema, the accumulation of...
1.0K
Renal Corpuscle01:20

Renal Corpuscle

9.5K
The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
9.5K
Pharmacogenomics: Identification of New Drug Targets01:29

Pharmacogenomics: Identification of New Drug Targets

91
Advances in genomics have profoundly influenced drug discovery by increasing both the speed and accuracy of pharmaceutical development. Pharmacogenomics, which examines how genetic variation influences drug response, facilitates the identification of novel therapeutic targets and enables patient stratification for personalized treatment. These strategies contribute to improved drug efficacy, minimized adverse effects, and more efficient clinical trial design.Mapping genetic differences...
91
Chronic Kidney Disease I: Introduction01:25

Chronic Kidney Disease I: Introduction

1.2K
Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage renal disease. At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate for...
1.2K
Nephrotic Syndrome II : Assessment and Medical Management01:26

Nephrotic Syndrome II : Assessment and Medical Management

355
IntroductionNephrotic syndrome is a kidney disorder marked by excessive protein loss in the urine, leading to various systemic complications. This condition often results from damage to the glomeruli—the kidney's filtering units—causing proteinuria, low blood protein levels, and fluid retention. Understanding the assessment, diagnosis, and management of nephrotic syndrome is essential for effective treatment and prevention of further kidney damage.AssessmentPatient History: Document...
355

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Readiness to Consider and Adopt Genetic and Genomic Tests in Canada-An Update to the State of Readiness Report.

Current oncology (Toronto, Ont.)·2026
Same author

Interferon-Inducible Gene Upregulation Correlates With Successful Viral Clearance in Patients With BK Polyomavirus-Associated Nephropathy.

Kidney international reports·2026
Same author

Corrigendum to "Molecular diagnosis of kidney allograft rejection based on the Banff Human Organ Transplant gene panel: A multicenter international study" [American Journal of Transplantation 25 (2025) 1631-1642].

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same author

Single-cell genomic analysis of cancer cells from one treatment-naïve patient with metastatic prostate cancer.

BMC genomic data·2026
Same author

Clazakizumab in the treatment of chronic active antibody-mediated kidney transplant rejection: Results from the IMAGINE phase 3, randomized, double-blind, placebo-controlled study.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same author

Normalization of nCounter Gene Expression Data Alters Molecular Diagnostics in Kidney Transplantation.

Journal of the American Society of Nephrology : JASN·2026

Related Experiment Video

Updated: Apr 11, 2026

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

832

Molecular nephropathology: ready for prime time?

Benjamin Adam1, Michael Mengel2

  • 1Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada.

American Journal of Physiology. Renal Physiology
|May 29, 2015
PubMed
Summary

Molecular diagnostics are crucial for precision nephropathology. The NanoString system enables transcriptomics on standard formalin-fixed tissues, overcoming barriers to integrating molecular insights into patient care.

Keywords:
NanoStringgene expressionmolecularnCounternephropathology

More Related Videos

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes
11:47

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Published on: March 4, 2022

2.7K
Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring
07:35

Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring

Published on: June 23, 2015

12.1K

Related Experiment Videos

Last Updated: Apr 11, 2026

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

832
Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes
11:47

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Published on: March 4, 2022

2.7K
Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring
07:35

Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring

Published on: June 23, 2015

12.1K

Area of Science:

  • Nephropathology
  • Molecular Diagnostics
  • Genomics

Background:

  • Current nephropathology relies on traditional microscopy, which is becoming insufficient for precision medicine.
  • Patient-specific molecular pathophysiology data is increasingly expected to supplement diagnoses.
  • Next-generation sequencing, particularly transcriptomics, is key for future molecular testing in nephropathology.

Purpose of the Study:

  • To address the technical barrier of fresh tissue requirements for molecular techniques in nephropathology.
  • To introduce a viable method for routine transcriptomics in clinical nephropathology.
  • To facilitate the translation of transcriptomics discoveries into improved patient care.

Main Methods:

  • Utilizing the NanoString gene expression system.
  • Working with formalin-fixed paraffin-embedded (FFPE) tissue samples, a standard in pathology.
  • Overcoming the limitation of requiring fresh tissue for molecular analysis.

Main Results:

  • The NanoString system demonstrates feasibility with FFPE tissues, a significant advancement.
  • This technology offers a pathway to integrate transcriptomics into routine nephropathology diagnostics.
  • Potential to enhance patient care through molecular insights.

Conclusions:

  • The NanoString system provides a promising solution for incorporating transcriptomics into clinical nephropathology.
  • Multidisciplinary collaboration and validation are essential for widespread adoption.
  • This approach bridges the gap between molecular discoveries and practical patient management in kidney disease.