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Updated: Apr 11, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
Published on: November 1, 2019
Production of a new non-specific nuclease from Yersinia enterocolitica subsp. palearctica: optimization of induction
Xiu-Juan Fang1, Zhen-Xing Tang2, Zhen-Hua Li1
1College of Life and Environmental Sciences, Hangzhou Normal University , Hangzhou , Zhejiang , P.R. China.
Abstract:
A new non-specific nuclease from Yersinia enterocolitica subsp. palearctica (Y. NSN) was expressed in Escherichia coli (E. coli) BL 21 StarTM (DE3)plysS. Induction conditions, including isopropyl-β-D-thiogalactoside (IPTG) concentration, cell density (OD600), induction time and induction temperature, were optimized using response surface methodology. Statistical analysis of the results revealed that induction temperature and all the quadratic terms of variables had significant effects on enzyme activity of Y. NSN. The optimal induction conditions were as follows: 1.5 mmol/L IPTG, OD600 of 0.80, induction time of 20.5 h, and induction temperature of 32 °C. Under the optimized conditions, the highest enzyme activity could be obtained.
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