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Updated: Apr 11, 2026
Intra-Arterial Delivery of Neural Stem Cells to the Rat and Mouse Brain: Application to Cerebral Ischemia
Published on: June 26, 2020
Combination cell therapy with mesenchymal stem cells and neural stem cells for brain stroke in rats
Seyed Mojtaba Hosseini1, Mohammad Farahmandnia2, Zahra Razi2
1Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran ; Cell and Molecular Student Research Group, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran ; Stem Cell laboratory, Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.
Objectives:
Brain stroke is the second most important events that lead to disability and morbidity these days. Although, stroke is important, there is no treatment for curing this problem. Nowadays, cell therapy has opened a new window for treating central nervous system disease. In some previous studies the Mesenchymal stem cells and neural stem cells. In this study, we have designed an experiment to assess the combination cell therapy (Mesenchymal and Neural stem cells) effects on brain stroke.
Method And Materials:
The Mesenchymal stem cells were isolated from adult rat bone marrow and the neural stem cells were isolated from ganglion eminence of rat embryo 14 days. The Mesenchymal stem cells were injected 1 day after middle cerebral artery occlusion (MCAO) and the neural stem cells transplanted 7 day after MCAO. After 28 days, the neurological outcomes and brain lesion volumes were evaluated. Also, the activity of Caspase 3 was assessed in different groups.
Result:
The group which received combination cell therapy had better neurological examination and less brain lesion. Also the combination cell therapy group had the least Caspase 3 activity among the groups.
Conclusions:
The combination cell therapy is more effective than Mesenchymal stem cell therapy and neural stem cell therapy separately in treating the brain stroke in rats.
