Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum: <i>Teredinibacter haidensis</i> sp. nov., <i>Teredinibacter purpureus</i> sp. nov. and <i>Teredinibacter franksiae</i> sp. nov., marine, cellulolytic endosymbiotic bacteria isolated from the gills of the wood-boring mollusc <i>Bankia setacea</i> (Bivalvia: Teredinidae) and emended description of the genus <i>Teredinibacter</i>.

International journal of systematic and evolutionary microbiology·2026
Same author

Temporal variability is an inherent feature of the healthy canine microbiome assessed by full-length 16S rRNA gene sequencing.

Animal microbiome·2026
Same author

Illumina complete long read assay yields contiguous bacterial genomes from human gut metagenomes.

mSystems·2025
Same author

Comparative genomic and phenotypic description of <i>Escherichia ruysiae</i>: a newly identified member of the gut microbiome of the domestic dog.

Frontiers in microbiology·2025
Same author

Species-level characterization of the core microbiome in healthy dogs using full-length 16S rRNA gene sequencing.

Frontiers in veterinary science·2024
Same author

<i>Xanthomonas rydalmerensis</i> sp. nov., a non-pathogenic member of Group 1 <i>Xanthomonas</i>.

International journal of systematic and evolutionary microbiology·2024

Related Experiment Video

Updated: Apr 11, 2026

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples
09:26

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Published on: June 30, 2023

1.8K

Swabs to genomes: a comprehensive workflow.

Madison I Dunitz1, Jenna M Lang1, Guillaume Jospin1

  • 1UC Davis, Genome Center, USA.

Peerj
|May 29, 2015
PubMed
Summary
This summary is machine-generated.

This study presents a simplified workflow for microbial genome sequencing and analysis, making it accessible for labs with limited resources and bioinformatics expertise. The goal is to empower more researchers to conduct microbial genomics research.

Keywords:
BioinformaticsGenome assemblyGenome sequencingMicrobial genomicsWorkflow

More Related Videos

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
12:37

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Published on: April 14, 2016

40.7K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

12.4K

Related Experiment Videos

Last Updated: Apr 11, 2026

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples
09:26

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Published on: June 30, 2023

1.8K
Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
12:37

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Published on: April 14, 2016

40.7K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

12.4K

Area of Science:

  • Microbiology
  • Genomics
  • Bioinformatics

Background:

  • Microbial genome sequencing is increasingly accessible due to advances in molecular biology and computational tools.
  • Inexperience with bioinformatics and the variety of DNA library preparation and sequencing options present barriers for many researchers.
  • There is a need for a straightforward, comprehensive workflow for microbial genomics.

Purpose of the Study:

  • To design, test, and troubleshoot a simple, comprehensive workflow for microbial genome sequencing.
  • To enable research labs and classrooms with limited resources and bioinformatics experience to perform microbial genomics.
  • To provide a clear pathway from environmental sample collection to a published microbial genome.

Main Methods:

  • Development of a standardized DNA library preparation protocol.
  • Optimization of sequencing parameters for microbial genomes.
  • Implementation of user-friendly bioinformatics pipelines for genome assembly and analysis.
  • Validation of the workflow using environmental samples.

Main Results:

  • A validated, end-to-end workflow for microbial genome sequencing was established.
  • The workflow demonstrated successful genome assembly and basic analysis from environmental samples.
  • The protocol proved effective for labs with limited resources and bioinformatics expertise.

Conclusions:

  • The developed workflow simplifies microbial genome sequencing and analysis, broadening accessibility.
  • This resource empowers a wider range of laboratories and educational settings to engage in microbial genomics.
  • The study facilitates the generation and publication of microbial genomes from diverse sources.