Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

4.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.3K
MicroRNAs01:22

MicroRNAs

24.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.8K
MicroRNAs01:22

MicroRNAs

12.1K
12.1K
RNA Interference01:23

RNA Interference

28.8K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.8K
RNA Interference01:23

RNA Interference

7.9K
7.9K
Experimental RNAi02:15

Experimental RNAi

8.4K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Depression of tryptophan may contribute to adverse effects of naproxen.

Nature communications·2026
Same author

Degree of Cyclooxygenase-2 Inhibition Modulates Blood Pressure Response.

Hypertension (Dallas, Tex. : 1979)·2025
Same author

Predictors of Supplemental Opioid Use After Third Molar Extraction.

medRxiv : the preprint server for health sciences·2025
Same author

Degree of Cyclooxygenase-2 Inhibition Modulates Blood Pressure Response to Celecoxib and Naproxen.

medRxiv : the preprint server for health sciences·2024
Same author

Differential Impact In Vivo of Pf4-ΔCre-Mediated and Gp1ba-ΔCre-Mediated Depletion of Cyclooxygenase-1 in Platelets in Mice.

Arteriosclerosis, thrombosis, and vascular biology·2024
Same author

The Circadian Biology of Heart Failure.

Circulation research·2023
Same journal

Fibrocytes drive JAK2V617F-mutated myelofibrosis: pitavastatin reverses marrow fibrosis and anemia.

Blood·2026
Same journal

Identifying steroid-refractory aGVHD before it happens.

Blood·2026
Same journal

ELISA-negative HIT: antibody recognition and relevance.

Blood·2026
Same journal

EBV and immunodeficiency: the odd couple drawn to the brain.

Blood·2026
Same journal

A bone to pick with ferric carboxymaltose.

Blood·2026
Same journal

A step toward streamlining HIT diagnosis.

Blood·2026
See all related articles

Related Experiment Video

Updated: Apr 11, 2026

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

14.8K

microRNA represses macromolecule.

Tilo Grosser1

  • 1UNIVERSITY OF PENNSYLVANIA.

Blood
|May 30, 2015
PubMed
Summary
This summary is machine-generated.

Hyperglycemia increases von Willebrand factor (VWF) by activating the polyol pathway and reducing microRNA-24 (miR-24). This discovery offers new insights into blood clotting regulation under high blood sugar conditions.

More Related Videos

MicroRNA-based Regulation of Picornavirus Tropism
09:05

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

8.1K
Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

37.0K

Related Experiment Videos

Last Updated: Apr 11, 2026

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

14.8K
MicroRNA-based Regulation of Picornavirus Tropism
09:05

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

8.1K
Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

37.0K

Area of Science:

  • Hematology
  • Molecular Biology
  • Endocrinology

Background:

  • Hyperglycemia is a hallmark of diabetes and is associated with increased thrombotic risk.
  • Von Willebrand factor (VWF) plays a critical role in primary hemostasis and thrombosis.
  • Dysregulation of VWF expression and secretion contributes to pathological conditions.

Purpose of the Study:

  • To elucidate the molecular mechanisms by which hyperglycemia influences VWF expression and secretion.
  • To identify novel pathways involved in hyperglycemia-mediated VWF dysregulation.

Main Methods:

  • Investigated the role of the polyol pathway in regulating VWF.
  • Assessed the impact of hyperglycemia on microRNA-24 (miR-24) levels.
  • Utilized cell-based assays to examine VWF expression and secretion.

Main Results:

  • Hyperglycemia activates the polyol pathway.
  • Activation of the polyol pathway leads to the repression of miR-24.
  • Repression of miR-24 augments VWF expression and secretion.

Conclusions:

  • The polyol pathway and miR-24 repression represent a novel mechanism linking hyperglycemia to increased VWF.
  • Targeting this pathway may offer therapeutic strategies for managing hyperglycemia-associated thrombotic complications.