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Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
Published on: February 9, 2024
Design of enzyme-interfaced DNA logic operations (AND, OR and INHIBIT) with an assaying application for single-base
1Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China. malong@tust.edu.cn.
Abstract:
We devised AND, OR and INHIBIT logic gates. They were based on the enzyme-induced DNA base flipping mechanism, which caused significant local conformation changes in DNA. This can be monitored via photonic signals as outputs. We also provided a prototype of a built-in biosensor capable of distinguishing single-base mismatches in a DNA duplex.
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