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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
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Cell cycle progression is regulated by intertwined redox oscillators.
Jorgelindo da Veiga Moreira1, Sabine Peres2, Jean-Marc Steyaert3
1Ecole Polytechnique, LIX-UMR 7161, Palaiseau, France. daveiga@lix.polytechnique.fr.
Theoretical Biology & Medical Modelling
|May 30, 2015
Summary
Cell cycle progression involves metabolic shifts, including increased glycolysis in G1 and Pentose Phosphate Pathway activation. Cytosolic pH changes coordinate mitochondrial activity and cell metabolism.
Area of Science:
- Cell Biology
- Biochemistry
- Metabolic Regulation
Background:
- Eukaryotic cell cycle phases (G0, G1, S, G2) exhibit conserved phenomena.
- Cell cycle progression involves DNA, RNA, and protein synthesis, alongside lipid synthesis.
- G1 phase shows increased glycolysis, high NAD+/NADH ratio, and cytosolic pH fluctuations.
Purpose of the Study:
- To investigate the role of cytosolic pH in coordinating cell cycle metabolism.
- To understand the interplay between pH, mitochondrial activity, and redox cycles.
- To elucidate metabolic shifts during different cell cycle phases.
Main Methods:
- Analysis of metabolic changes during cell cycle progression.
- Monitoring cytosolic pH and transmembrane potential.
- Observing mitochondrial dynamics and ATP synthesis.
Main Results:
- G1 phase exhibits increased glycolysis, cytosolic acidification followed by alkalinization, and hyperpolarized membrane potential.
- Pentose Phosphate Pathway (PPP) is activated in conjunction with increased NADP+/NADPH ratio.
- Late G1/S transition shows cytosolic acidification, mitochondrial hyperfusion, and ATP synthesis via oxidative phosphorylation.
Conclusions:
- Cytosolic pH fluctuations may coordinate mitochondrial activity and redox cycles.
- These coordinated activities regulate overall cell metabolism during the cell cycle.
- Metabolic shifts, including pH changes, are crucial for cell cycle progression.
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