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Characterization of outer membrane vesicles from a neonatal meningitic strain of Cronobacter sakazakii

Hayat Alzahrani1, Jody Winter1, David Boocock2

  • 1Pathogen Research Group, School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK.

FEMS Microbiology Letters
|May 30, 2015
PubMed

Insights

Cronobacter sakazakii outer membrane vesicles (OMV) are produced by the bacteria and can activate inflammatory responses in human intestinal cells. These OMV may deliver toxins, contributing to the pathogen

Area of Science:

  • Microbiology
  • Pathogenesis
  • Cell Biology

Background:

  • Cronobacter sakazakii causes severe infant meningitis and necrotizing enterocolitis.
  • The cytopathogenic mechanisms of C. sakazakii and its interaction with host cells remain poorly understood.
  • The role of Cronobacter outer membrane vesicles (OMV) in pathogenesis has not been previously investigated.

Purpose of the Study:

  • To investigate the production and pathogenic functions of Cronobacter sakazakii outer membrane vesicles (OMV).
  • To elucidate the interaction of C. sakazakii OMV with human intestinal epithelial cells.
  • To identify proteins within C. sakazakii OMV and assess their potential roles in pathogenicity.

Main Methods:

  • Isolation and characterization of OMV from Cronobacter sakazakii strain 767.
  • Incubation of Caco-2 cells with C. sakazakii OMV.
  • Analysis of cellular responses including proliferation and inflammatory markers.
  • Mass spectrometry-based proteomic analysis of OMV-associated proteins.

Main Results:

  • Cronobacter sakazakii produces OMV that are internalized by Caco-2 cells.
  • OMV treatment increased cell proliferation and stimulated innate proinflammatory responses without causing overt toxicity.
  • Eighteen OMV-associated proteins were identified, suggesting potential roles in pathogenicity.

Conclusions:

  • Cronobacter sakazakii OMV are produced and internalized by intestinal epithelial cells.
  • OMV contribute to host cell responses, including proliferation and inflammation, potentially via delivered toxins.
  • These findings suggest a significant role for C. sakazakii OMV in the pathogenesis of C. sakazakii infections.
Keywords:
Cronobacter sakazakiicytopathogenicitymeningitisouter membrane vesiclesproteomic

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