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Updated: Apr 11, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
The mitochondrial genome of Papilio demoleus Linnaeus (Lepidoptera: Papilionidae)
Tianrong Xin1, Chengyi Yao1, Lei Li1
1a College of Life Science, Nanchang University , Nanchang , China.
Abstract:
We determined the complete mitochondrial genome (mitogenome) sequence of Papilio demoleus (GenBank accession number KR024009) by long PCR and primer walking methods. The total length of mitochondrial DNA is 15,249 bp containing 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. The overall base composition of the genome is A (39.31%), T (41.57%), C (11.33%) and G (7.78%) with an A + T-rich region, similar to other invertebrate mitochondrial genomes. The start codon was mainly ATG in most of the mitochondrial protein-coding genes such as COII, ATP6, COIII, ND4, ND4L, Cob and ND1, while ATA for ND2, COI, ATP8, ND3, ND5 and ND6 genes. The stop codon was mainly TAA in most of the mitochondrial protein-coding genes, whereas TAG was found in ND1 gene only. The A + T region is located between 12S rRNA and tRNA(M)(et) with a length of 403 bp.
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