Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease in membrane-bound G4 form of acetylcholinesterase in postmortem Alzheimer brain.

K M Schegg1, S Nielsen, R M Zweig

  • 1VA Medical Center, Reno.

Progress in Clinical and Biological Research
|January 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experience with Abdominal Aortic Aneurysims.

Bristol medico-chirurgical journal (1963)·2017
Same author

Identification of a calcitonin-like diuretic hormone that functions as an intrinsic modulator of the American lobster, Homarus americanus, cardiac neuromuscular system.

The Journal of experimental biology·2009
Same author

Istradefylline as monotherapy for Parkinson disease: results of the 6002-US-051 trial.

Parkinsonism & related disorders·2009
Same author

Identification of proteins electroblotted to polyvinylidene difluoride membrane by combined amino acid analysis and bioinformatics: An ABRF multicenter study.

Journal of biomolecular techniques : JBT·2009
Same author

DBS and diathermy interaction induces severe CNS damage.

Neurology·2001
Same author

Immunocytochemical and immunoelectron microscopic localization of alpha-, beta-, and gamma-ENaC in rat kidney.

American journal of physiology. Renal physiology·2001

Alzheimer's disease (AD) is linked to reduced choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activity in specific brain regions. The study found a decrease in membrane-bound AChE forms, suggesting a regulatory factor may be involved in AD pathogenesis.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Pathology

Background:

  • Alzheimer's disease (AD) is a neurodegenerative disorder characterized by cognitive decline.
  • Acetylcholine deficiency is a hallmark of AD, impacting neurotransmission.
  • Acetylcholinesterase (AChE) and choline acetyltransferase (ChAT) are key enzymes in acetylcholine metabolism.

Purpose of the Study:

  • To investigate the specific activities and molecular forms of ChAT and AChE in brain regions affected by Alzheimer's disease.
  • To compare enzyme activity and composition in Alzheimer's disease patients, controls, and non-Alzheimer's dementia patients.
  • To explore the relationship between enzyme activity and AChE molecular form composition in Alzheimer's disease.

Main Methods:

  • Post-mortem brain tissue samples from hippocampus, septal nucleus, area 40, and nucleus basalis of Meynert were analyzed.

Related Experiment Videos

  • Specific activities of ChAT and AChE were measured using biochemical assays.
  • AChE molecular form composition, particularly the globular tetrameric (G4) form and its membrane-bound fraction, was determined.

    • Main Results:

    • Significantly lower ChAT activity was observed in the hippocampus, septum, and area 40 of AD patients compared to controls.
    • AChE specific activity was reduced in the hippocampus and area 40 of AD patients.
    • A decrease in the percentage of membrane-bound G4 AChE was significant in the hippocampus and area 40 of AD patients.

    Conclusions:

    • Reduced ChAT and altered AChE molecular forms, specifically decreased membrane-bound G4, are characteristic biochemical changes in Alzheimer's disease brain regions.
    • The findings suggest a potential soluble factor regulating membrane-bound G4 AChE production, which may be disrupted in AD.
    • These enzymatic alterations provide insights into the neurochemical basis of Alzheimer's disease pathology.