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Updated: Apr 11, 2026
Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory
Published on: January 1, 2018
Analysis of estrogenic activity in environmental waters in Rio de Janeiro state (Brazil) using the yeast estrogen
Amanda Cristina Vieira Dias1, Frederico Wegenast Gomes1, Daniele Maia Bila2
1Chemical Engineering Program - COPPE, Federal University of Rio de Janeiro, P.O. Box 68502, 21941-972 Rio de Janeiro, RJ, Brazil.
Abstract:
The estrogenicity of waters collected from an important hydrological system in Brazil (Paraiba do Sul and Guandu Rivers) was assessed using the yeast estrogen screen (YES) assay. Sampling was performed in rivers and at the outlets of conventional water treatment plants (WTP). The removal of estrogenic activity by ozonation and chlorination after conventional water treatment (clarification and sand filtration) was investigated employing samples of the Guandu River spiked with estrogens and bisphenol A (BPA). The results revealed a preoccupying incidence of estrogenic activity at levels higher than 1ngL(-1) along some points of the rivers. Another matter of concern was the number of samples from WTPs presenting estrogenicity surpassing 1ngL(-1). The oxidation techniques (ozonation and chlorination) were effective for the removal of estrogenic activity and the combination of both techniques led to good results using less amounts of oxidants.
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