Related Experiment Video
Updated: Apr 11, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Description of Geodermatophilus bullaregiensis sp. nov
Karima Hezbri1, Faten Ghodhbane-Gtari, Maria Del Carmen Montero-Calasanz
1Laboratoire Microorganismes et Biomolécules Actives, Université de Tunis El Manar (FST) & Université de Carthage (INSAT), 2092, Tunis, Tunisia.
Abstract:
The taxonomic position of an aerobic actinobacterial strain, BMG841(T), isolated from the Bulla Regia monument (Tunisia) and exhibiting a high resistance to gamma-radiation (D10 ~9 kGy) was determined using polyphasic approach. The optimal growth range was found to be 25-35 °C at pH of 7.0-8.5. The strain was observed to form black dry colonies. Chemotaxonomic characteristics of the isolate showed a cell wall type III, with galactose and glucose as diagnostic sugars; phosphatidylcholine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid as main polar lipids; and MK-9(H4) as the predominant menaquinone. The major cellular fatty acids were identified as iso-C16:0 and iso-C15:0. Phylogenetic analysis indicated that strain BMG841(T) represents a novel member of the genus Geodermatophilus with high 16S rRNA gene sequence identity with Geodermatophilus saharensis (98.28 %). Based on phylogenetic and phenotypic analysis, strain BMG841(T) is proposed as the type strain (=DSM 46841(T) = CECT 8821(T)) of a novel species, Geodermatophilus bullaregiensis.
Related Concept Videos
Bacterial Phylum Spirochaetes
Hyperthermophilic Bacteria
Diversity of Protists I
Bacterial Phylum Verrucomicrobiota
Bacterial Phylum Actinobacteria
Bacterial Phylum Planctomycetes

