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Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
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Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
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Related Experiment Video

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Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
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RPdb: a database of experimentally verified cellular reprogramming records.

Yi Shen1, Fan Gao2, Minghui Wang3

  • 1School of Information Science and Technology, University of Science and Technology of China, Hefei, AH230027, China.

Bioinformatics (Oxford, England)
|May 31, 2015
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Summary

Researchers created RPdb, a comprehensive database of cellular reprogramming. This resource integrates information from over 2000 articles, aiding regenerative medicine and cell biology studies.

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Area of Science:

  • Cellular reprogramming and regenerative medicine.
  • Stem cell research and differentiation potential.

Background:

  • Cellular reprogramming methods, including induced pluripotent stem cells (IPSCs), are crucial for regenerative medicine.
  • Direct reprogramming of somatic cells bypasses stem cell stages, offering alternative therapeutic avenues.
  • Existing literature lacks a centralized, integrated database for reprogramming records.

Purpose of the Study:

  • To establish a dedicated database for cellular reprogramming information.
  • To facilitate access to curated reprogramming data for researchers.
  • To support the advancement of regenerative medicine and cell biology.

Main Methods:

  • Manual extraction of data from over 2000 published scientific articles.
  • Development of the RPdb (cellular reprogramming database).

Main Results:

  • RPdb successfully integrates diverse cellular reprogramming information.
  • The database provides easy access to manually curated reprogramming records.
  • RPdb serves as a valuable resource for researchers in regenerative medicine and cell biology.

Conclusions:

  • The establishment of RPdb addresses the need for a centralized repository of cellular reprogramming data.
  • RPdb enhances accessibility to vital information for advancing research in regenerative medicine and cell biology.
  • This database supports the ongoing search for novel reprogramming strategies and mechanistic understanding.