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SDF-1/CXCR4 promotes F5M2 osteosarcoma cell migration by activating the Wnt/β-catenin signaling pathway

Yao Lu1, Bin Hu, Guo-Feng Guan

  • 1Department of Orthopaedic Surgery, Tangdu Hospital, The Fourth Military Medical University, 569 Xin Si Road, Xi'an, 710038, Shaanxi, China.

Medical Oncology (Northwood, London, England)
|June 1, 2015
PubMed

Insights

Stromal cell-derived factor (SDF-1) promotes osteosarcoma (OS) cell migration by activating the Wnt/β-catenin pathway. Targeting the SDF-1/CXCR4 axis may offer a new therapeutic strategy for this bone cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Osteosarcoma (OS) is a primary malignant bone tumor in children and adolescents with limited effective treatments.
  • The stromal cell-derived factor (SDF-1)/CXCR4 axis is implicated in tumor cell migration, proliferation, and survival.

Purpose of the Study:

  • To investigate the role of SDF-1/CXCR4 signaling in mediating chemotaxis in F5M2 OS cells.
  • To elucidate the underlying molecular mechanisms involved in SDF-1-induced OS cell migration.

Main Methods:

  • Immunohistochemistry and immunofluorescence microscopy for protein expression.
  • Real-time quantitative polymerase chain reaction for RNA expression.
  • Western blotting for protein analysis and migration assays.

Main Results:

  • CXCR4 and β-catenin mRNA and protein expression were significantly elevated in OS tissues compared to non-neoplastic tissues.
  • SDF-1 significantly promoted F5M2 cell migration.
  • SDF-1-induced migration was dependent on the activation of AKT and Wnt/β-catenin signaling pathways.

Conclusions:

  • The SDF-1/CXCR4 axis promotes F5M2 osteosarcoma cell migration.
  • This migratory process is regulated by the Wnt/β-catenin signaling pathway.
  • Inhibition of SDF-1/CXCR4 signaling abrogated SDF-1-induced migration, suggesting therapeutic potential.

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