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Updated: Apr 11, 2026
Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
[Isolation and identification of an antimicrobial endophytic actinomycete from Macleaya cordata]
Objective:
An antimicrobial endophytic actinomycete strain was isolated from the root of Macleaya cordata, and its phylogenetic position was also investigated.
Methods:
Endophytic actinomycetes were isolated from the root of Macleaya cordata growing in Dabie Mountain by tablet coating method. Antimicrobial activities of endophytic actinomycetes were determined by the agar block method,and then a potential strain was identified by morphology, biochemical characterization and molecular methods.
Results:
A total of 15 strains of endophytic actinomycetes were isolated from the samples, six of them had antimicrobial activity to the test strains which accounts for 40% of all strains. Among the six strains,the strain BL7 was found to produce antibiotic substances which showed significant inhibitory effects on Staphylococcus aureus and Bacillus subtilis,with a formed inhibition zone of 29 mm and 20 mm respectively. Based on the morphology, biochemical characterization and 16S rDNA sequence analysis,the strain BL7 was preliminary identified as Streptomyces mobaraensis.
Conclusion:
Some members of Streptomyces genus have a very wide application prospect in medicine. This is the first report that Streptomyces mobaraensis strain was isolated from Macleaya cordata, and this study provides basis for the further exploration of the strain.
