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Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
Published on: August 26, 2016
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Co-extraction of DNA and PLFA from soil samples
Sheridan Brewer1, Stephen M Techtmann2, Nagissa Mahmoudi2
1Department of Microbiology, College of Arts & Sciences, University of Tennessee, Knoxville, TN 37996, United States.
Journal of Microbiological Methods
|June 2, 2015
Abstract:
Lipid/DNA co-extraction from one sample is attractive in limiting biases associated with microbial community analysis from separate extractions. We sought to enhance established co-extraction methods and use high-throughput 16S rRNA sequencing to identify preferentially extracted taxa from co-extracted DNA. Co-extraction results in low DNA yields and distinct community structure changes.
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