Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Genetic Engineering?00:49

What is Genetic Engineering?

81.7K
Overview
81.7K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

17.7K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
17.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NELFA supports naïve pluripotency and drives 8C-like state in human embryonic stem cells.

Nature communications·2026
Same author

A post-implantation model of human embryo development includes a definitive hematopoietic niche.

Cell reports·2025
Same author

The emergence of human primordial germ cell-like cells in stem cell-derived gastruloids.

Science advances·2025
Same author

Stem cell-based embryo models: a tool to study early human development.

Science China. Life sciences·2025
Same author

The role of KLF4 in human primordial germ cell development.

Open biology·2025
Same author

Temporal and regional X-linked gene reactivation in the mouse germline reveals site-specific retention of epigenetic silencing.

Nature structural & molecular biology·2025

Related Experiment Video

Updated: Apr 11, 2026

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
09:37

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

Published on: March 5, 2017

13.7K

Human Germline: A New Research Frontier.

M Azim Surani1

  • 1Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK; Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3EG, UK; Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 3EG, UK.

Stem Cell Reports
|June 2, 2015
PubMed
Summary
This summary is machine-generated.

Human primordial germ cell (hPGC) specification involves epigenetic reprogramming, differing from mouse models. Specific DNA demethylation-resistant loci in the human germline may influence transgenerational epigenetic inheritance and disease.

More Related Videos

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
05:48

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

3.1K
Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
12:04

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

5.2K

Related Experiment Videos

Last Updated: Apr 11, 2026

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
09:37

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

Published on: March 5, 2017

13.7K
Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
05:48

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

3.1K
Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
12:04

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

5.2K

Area of Science:

  • Developmental Biology
  • Epigenetics
  • Human Germline Development

Background:

  • Human primordial germ cell (hPGC) specification and epigenetic reprogramming are crucial for development.
  • Understanding these processes is vital for insights into human pluripotency and early development.
  • Previous studies often relied on mouse models, highlighting the need for human-specific investigations.

Purpose of the Study:

  • To elucidate the mechanism of hPGC specification and epigenetic resetting in humans.
  • To identify differences in epigenetic regulation between human and mouse germline development.
  • To investigate loci resistant to DNA demethylation in the early human germline.

Main Methods:

  • Analysis of epigenetic modifications during hPGC specification in vivo.
  • Comparative studies with mouse models to identify species-specific regulatory mechanisms.
  • Identification and characterization of DNA demethylation-resistant loci.

Main Results:

  • hPGC specification regulators initiate comprehensive epigenetic resetting, including DNA demethylation, imprinting erasure, and X reactivation.
  • Significant differences observed compared to mouse models, likely due to variations in pluripotency regulation and gastrulation.
  • Identified specific loci resistant to DNA demethylation in the hypomethylated early human germline, predominantly expressed in neural cells.

Conclusions:

  • Epigenetic reprogramming in hPGCs is a complex process with unique features in humans.
  • Non-rodent models are essential for accurate investigation of human developmental processes.
  • Demethylation-resistant loci offer a model for studying transgenerational epigenetic inheritance and environmental influences on development and disease.