Related Experiment Video

Updated: Apr 11, 2026

Techniques to Induce and Quantify Cellular Senescence
06:51

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

35.5K

Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG

Liwei Ma1, Wenting Zhao1, Feng Zhu1

  • 1Research Center on Aging. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing Key Laboratory of Protein Posttranslational Modifications and Cell Function , Beijing , China.

Frontiers in Endocrinology
|June 2, 2015
PubMed

Insights

Cellular senescence-inhibited gene (CSIG), or RSL1D1, regulates cell cycle and senescence. Gene expression profiling identified CSIG targets, revealing its role in transcriptional and post-transcriptional regulation.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genomics

Background:

  • Cellular senescence-inhibited gene (CSIG), also known as RSL1D1, is involved in cell cycle regulation, senescence, apoptosis, and tumor metastasis.
  • The precise regulatory mechanisms governing CSIG's functions remain largely uncharacterized.

Purpose of the Study:

  • To identify key target genes and signaling pathways modulated by CSIG.
  • To elucidate the molecular mechanisms underlying CSIG's role in cell proliferation and senescence.

Main Methods:

  • Gene expression profiling using Affymetrix microarray in CSIG-silencing and control HEK293 cells.
  • Validation of differentially expressed genes using real-time PCR and western blot analysis.
  • Analysis of CSIG expression patterns with cell cycle and senescence markers, and investigation of mRNA half-life modulation.

Main Results:

  • CSIG silencing resulted in significant changes in 590 genes, with 279 up-regulated and 311 down-regulated.
  • Affected genes are involved in transcriptional regulation, cell cycle, signal transduction, oxidation-reduction, development, and cell adhesion.
  • CSIG was found to modulate the mRNA half-life of specific genes (Cdc14B, CASP7, CREBL2) and its expression correlates with cell cycle and senescence progression.

Conclusions:

  • Gene expression profiling is effective for identifying CSIG-regulated genes at both transcriptional and post-transcriptional levels.
  • CSIG plays a significant role in regulating cell proliferation and senescence through modulation of gene expression and mRNA stability.
Keywords:
CSIG/RSL1D1cell cyclegene expressionmicroarraysenescence

More Related Videos

Evaluating the Effect of SASP Factors on the Proliferation of Cancer Cells Using a Comparative Analysis of Three Distinct Methodologies08:57

Evaluating the Effect of SASP Factors on the Proliferation of Cancer Cells Using a Comparative Analysis of Three Distinct Methodologies

Published on: September 19, 2025

763
Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis07:26

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

1.2K

Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
4.4K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
10.4K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.3K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.9K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.2K