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Related Concept Videos

Bioreactor Controls-II01:18

Bioreactor Controls-II

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In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the...
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Bioreactor Design and Operational System01:29

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Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
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Scale-Up Processes01:14

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The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
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Bioreactor Controls-III01:22

Bioreactor Controls-III

54
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
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Related Experiment Video

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Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site
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Simplifying microbial electrosynthesis reactor design.

Cloelle G S Giddings1, Kelly P Nevin2, Trevor Woodward2

  • 1Department of Civil and Environmental Engineering, University of Massachusetts at Amherst Amherst, MA, USA.

Frontiers in Microbiology
|June 2, 2015
PubMed
Summary
This summary is machine-generated.

Microbial electrosynthesis, an artificial photosynthesis, can be simplified. Researchers developed a membrane-less reactor using a direct current power source, maintaining high efficiency for converting carbon dioxide into valuable organic compounds.

Keywords:
CO2 sequestrationSporomusa ovataartificial photosynthesisbioelectrochemical systemmicrobial electrosynthesisrenewable energy storage

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Area of Science:

  • Biotechnology
  • Electrochemistry
  • Synthetic Biology

Background:

  • Microbial electrosynthesis (MES) mimics photosynthesis to convert CO2 into organic chemicals.
  • Traditional MES reactors are complex and pose scale-up challenges.
  • Sporomusa ovata is a microbe capable of reducing CO2 to acetate.

Purpose of the Study:

  • To simplify MES reactor design by removing potentiostatic control and membranes.
  • To investigate the feasibility of using a direct current power source instead of potentiostatic control.
  • To assess the efficiency of a membrane-less MES reactor with S. ovata.

Main Methods:

  • Utilized Sporomusa ovata biofilms on graphite electrodes.
  • Compared traditional H-cell reactors with a simplified membrane-less design.
  • Employed a direct current power source for cathode electron delivery.
  • Monitored acetate production, coulombic efficiency, and energetic efficiency.

Main Results:

  • High rates and coulombic efficiencies were maintained in a membrane-less reactor with direct current power.
  • Eliminating the membrane and potentiostatic control did not hinder MES performance.
  • The simplified reactor design successfully produced acetate from CO2 with high efficiency.

Conclusions:

  • MES is feasible in simplified, membrane-less reactors powered by direct current.
  • This approach significantly reduces reactor complexity and potential construction costs.
  • The findings pave the way for more scalable and cost-effective MES applications.