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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
Identification of fleeting electrochemical reaction intermediates using desorption electrospray ionization mass
Timothy A Brown1, Hao Chen2, Richard N Zare1
1‡Department of Chemistry, Stanford University, Stanford, California 94305-5080, United States.
Abstract:
We report a new method for the mass spectrometric detection of fleeting reaction intermediates in electrochemical reactions utilizing a "waterwheel" working electrode setup. This setup takes inspiration from desorption electrospray ionization (DESI) mass spectrometry, where the sampling time is on the order of milliseconds, to sample directly from the surface of a working electrode for mass spectrometric analysis. We present data that show the formation of a diimine intermediate of the electrochemical oxidation of uric acid that has a lifetime in solution of 23 ms as well as data that provide evidence for the formation of a similar diimine species from the electrooxidation of xanthine, which has not been previously observed.
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