Novel process for producing 6-deoxy monosaccharides from l-fucose by coupling and sequential enzymatic method

Area of Science:

• Carbohydrate Chemistry
• Enzymatic Synthesis
• Biotechnology

Background:

• Deoxy sugars are rare in nature, limiting their availability for research and applications.
• L-fucose is a readily available starting material for deoxy sugar synthesis.
• Enzymatic transformations offer precise and efficient routes for carbohydrate modification.

Purpose of the Study:

• To develop novel enzymatic methods for the biosynthesis of rare 6-deoxy sugars.
• To produce 6-deoxy-L-talose, 6-deoxy-L-sorbose, 6-deoxy-L-gulose, and 6-deoxy-L-idose from L-fucose.
• To optimize reaction conditions for efficient deoxy sugar production.

Main Methods:

• Coupling and sequential enzymatic reactions using immobilized isomerases and epimerases.
• Utilized D-arabinose isomerase, L-rhamnose isomerase, D-allulose 3-epimerase, L-ribose isomerase, and D-glucose isomerase.
• One-pot and sequential reaction strategies were employed for product synthesis.

Main Results:

• Successfully biosynthesized 6-deoxy-L-talose, 6-deoxy-L-sorbose, 6-deoxy-L-gulose, and 6-deoxy-L-idose from L-fucose.
• Achieved production yields of 7.1% for 6-deoxy-L-talose, 14% for 6-deoxy-L-sorbose, 2% for 6-deoxy-L-gulose, and 2.4% for 6-deoxy-L-idose.
• Established specific enzymatic pathways for the targeted synthesis of each deoxy sugar.

Conclusions:

• Demonstrated the feasibility of enzymatic synthesis for producing rare deoxy sugars from L-fucose.
• Developed efficient enzymatic cascades for the production of 6-deoxy sugars.
• The study expands the synthetic accessibility of uncommon carbohydrate structures.