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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Related Experiment Video

Updated: Apr 11, 2026

Author Spotlight: Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification
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Targeted genome editing in primate embryos.

Xiangyu Guo1, Xiao-Jiang Li2

  • 1Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

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|June 3, 2015
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Summary
This summary is machine-generated.

CRISPR/Cas9 gene editing faces challenges with mosaic mutations and off-target effects in non-human primates. These technical issues must be addressed for creating accurate primate models of human diseases.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Primate Research

Background:

  • CRISPR/Cas9 is a powerful gene-editing tool.
  • Concerns exist regarding its efficiency and specificity in large animals, particularly non-human primates.
  • Mosaic mutations and off-target effects are key issues.

Purpose of the Study:

  • To discuss recent findings on CRISPR/Cas9 in primate embryos.
  • To highlight technical challenges in using CRISPR/Cas9 for disease modeling in non-human primates.

Main Methods:

  • Review of recent findings in primate embryo research.
  • Focus on technical aspects of CRISPR/Cas9 application.

Main Results:

  • CRISPR/Cas9 application in primate embryos results in mosaic mutations.
  • Off-target mutations are observed, impacting specificity.
  • Technical hurdles remain for efficient and precise gene editing.

Conclusions:

  • CRISPR/Cas9 efficiency and specificity are critical concerns for non-human primate research.
  • Addressing mosaicism and off-target effects is essential for developing reliable primate models of human diseases.