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Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Updated: Apr 11, 2026

The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior

The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior

Published on: June 12, 2019

Culturing mouse tumor cells.

Andrew D Rhim¹, Martin Jechlinger², Anil K Rustgi³

¹Division of Gastroenterology, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109;

Cold Spring Harbor Protocols

|June 3, 2015

Summary

This summary is machine-generated.

Genetically engineered mouse models (GEMMs) are valuable for cancer research but costly. In vitro culture systems offer an efficient alternative or complement for studying tumor cells and testing hypotheses, especially for carcinomas.

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Last Updated: Apr 11, 2026

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Published on: June 12, 2019

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Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model

Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model

Published on: October 9, 2015

Area of Science:

Oncology
Cell Biology
Genetics

Background:

Genetically engineered mouse models (GEMMs) are crucial for cancer research.
GEMMs present challenges in terms of time and cost for production and maintenance.
In vitro culture systems can serve as a valuable alternative or complementary approach.

Purpose of the Study:

To introduce the utility of in vitro culture systems for cancer research.
To discuss the challenges associated with in vitro analyses of primary cancer cells.
To highlight best practice protocols for working with diverse carcinoma types in vitro.

Main Methods:

Review of existing literature on in vitro cancer cell culture.
Discussion of challenges in primary cancer cell isolation and culture.
Presentation of optimized protocols for various carcinoma types.

Main Results:

In vitro systems can efficiently test hypotheses related to cancer biology.
These systems complement findings from GEMMs.
Best practice protocols enhance the reliability of in vitro carcinoma studies.

Conclusions:

In vitro culture systems provide an efficient and effective method for cancer research.
Careful protocol selection is essential for successful in vitro analyses of carcinomas.
These methods can accelerate cancer research by complementing GEMM studies.