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In vivo Imaging Method to Distinguish Acute and Chronic Inflammation

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Published on: August 16, 2013

Lysosomal pH Decrease in Inflammatory Cells Used To Enable Activatable Imaging of Inflammation with a Sialic Acid

Mingzhu Yu¹, Xuanjun Wu¹, Bijuan Lin¹

¹†Department of Chemical Biology, College of Chemistry and Chemical Engineering, State Key Laboratory for Physical Chemistry of Solid Surfaces, the Key Laboratory for Chemical Biology of Fujian Province, the MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, and Innovation Center for Cell Signaling Network, and ‡State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, 361005, China.

Analytical Chemistry

|June 3, 2015

Summary

Researchers developed a novel imaging agent that targets acidic lysosomes in inflammatory cells. This approach enables dual-modality imaging of inflammation, offering a new biomarker for disease detection.

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Published on: January 5, 2015

Area of Science:

Biomedical Imaging
Molecular Imaging
Inflammation Biology

Background:

Inflammation is a major cause of disease and death, requiring advanced in vivo imaging techniques.
Current imaging methods often use optical agents targeting protein biomarkers, with limitations in specificity and sensitivity.
There is a need for novel strategies to accurately visualize and monitor inflammatory processes in real-time.

Purpose of the Study:

To introduce a new imaging approach for inflammation by targeting acidic lysosomes within inflammatory cells.
To develop and evaluate a sialic acid (Sia) conjugated near-infrared profluorophore (pNIR) for inflammation imaging.
To demonstrate the potential of lysosomes as a previously unrecognized biomarker for inflammation imaging.

Main Methods:

Synthesis of Sia-pNIR, a probe with a sialic acid targeting moiety and a pH-sensitive near-infrared profluorophore.
In vivo administration of Sia-pNIR in mouse models of inflammation induced by bacteria (E. coli, S. aureus) or lipopolysaccharide (LPS).
Evaluation of Sia-pNIR performance using fluorescence and optoacoustic imaging, assessing signal contrast and response to antibiotic treatment.

Main Results:

Sia-pNIR demonstrated high signal contrast between inflamed and healthy tissues in various inflammation models.
The probe's fluorescence signal was effectively switched off following antibiotic treatment, indicating responsiveness to inflammation resolution.
Acidic lysosomes within inflammatory cells were confirmed as a viable and previously unappreciated target for imaging.

Conclusions:

Sia-pNIR represents a novel activatable probe for dual-modality inflammation imaging.
Targeting acidic lysosomes in inflammatory cells offers a new strategy for sensitive and specific inflammation detection.
This approach holds promise for improved monitoring of inflammatory diseases and treatment response.