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Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity
Published on: June 7, 2017
Characterization of activated peritoneal cells inhibiting the take of transplantable murine tumours
J Minárovits1, E Karczag, I Földes
1National Institute of Hygiene, Microbiological Research Group, Budapest, Hungary.
Abstract:
We studied the properties of activated peritoneal cells (PC) inhibiting the take of SP4 spontaneous adenocarcinoma and Lewis lung carcinoma in syngeneic mice. Treatment of the poly I:C activated PC from Balb/c mice suppressing the take of SP4 tumour with anti-asialo GM1 antibody and complement before transfer did not affect their tumour-inhibitory potential. PC from Balb/c nude mice treated with poly I:C also inhibited the take of SP4 tumour. Spleen cells from untreated or poly I:C treated Balb/c and Balb/c nude mice, however, did not inhibit the take of SP4 adenocarcinoma. Treatment of peritoneal cells activated by a combination of poly I:C, indomethacin and Syncumar (referred to as "combined treatment") with anti-asialo GM1 antibody and complement could not, or could only partly abolish their tumour-inhibitory potential. The cells mediating the suppression of the take of Lewis lung tumour proved to be Thy-1,2+/-, Lyt-1-, Lyt 2.2- cells. We conclude that the activated peritoneal cells inhibiting the take of SP4 adenocarcinoma and Lewis lung tumour are different from NK cells, NC cells and LAK cells and represent a distinct antitumoural effector cell population.
Insights
Activated peritoneal cells (PC) show potent anti-tumor properties against SP4 adenocarcinoma and Lewis lung carcinoma in mice. These cells represent a distinct antitumoral effector population, separate from NK, NC, and LAK cells.
Area of Science:
- Immunology
- Cancer Research
- Cell Biology
Background:
- Peritoneal cells (PC) play a role in immune responses.
- Understanding tumor-rejection mechanisms is crucial for cancer therapy.
Purpose of the Study:
- To investigate the properties of activated peritoneal cells (PC) that inhibit tumor growth.
- To characterize the effector cell population responsible for tumor suppression.
Main Methods:
- Activation of peritoneal cells using poly I:C, indomethacin, and Syncumar.
- Inhibition assays using SP4 spontaneous adenocarcinoma and Lewis lung carcinoma in syngeneic mice.
- Characterization of effector cells using antibody and complement treatments (anti-asialo GM1) and cell surface marker analysis (Thy-1,2, Lyt-1, Lyt 2.2).
Main Results:
- Activated PC from Balb/c and Balb/c nude mice inhibited tumor take.
- Treatment with anti-asialo GM1 antibody and complement did not abolish tumor-inhibitory potential.
- Spleen cells did not inhibit tumor take.
- The effector cells were identified as Thy-1,2+/-, Lyt-1-, Lyt 2.2-.
Conclusions:
- Activated peritoneal cells represent a distinct antitumoral effector cell population.
- These cells are different from NK cells, NC cells, and LAK cells.
- The study identifies a novel cell population with potential in cancer immunotherapy.
